Application
Western blot analysis of SEPHS2 expression in K-562 whole cell lysate.Application
Western blot analysis of SEPHS2 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.Application
Western blot analysis of SEPHS2 expression in non-transfected 293T: (A), human SEPHS2 transfected 293T: (B) and K-562 (C) whole cell lysates.| Product Name | SEPHS2 Antibody (I-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SEPHS2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | SEPHS2 (selenophosphate synthetase 2), also known as SPS2, Selenium donor protein 2 or Selenide water dikinase 2, is a member of the selenophosphate synthetase 1 family of proteins. SEPHS2 is one of two mammalian homologs of the eubacteria selenophosphate synthetase protein SelD. SelD is an enzyme that generates the selenium donor for the biosynthesis of selenocysteine. SEPHS2 has a similar function to SelD and specifically catalyzes the formation of selenophosphate (the active selenium donor) from selenide and ATP. The knockdown of SEPHS2 expression greatly impairs selenoprotein biosynthesis, suggesting that SEPHS2 is the mammalian enzyme responsible for generating the selenium donor and regulating selenoprotein synthesis. In addition, SEPHS2 is itself a selenoprotein, implying that it may also function as an auto-regulator. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SEPHS2 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SEPHS2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 22928 |
|---|
Application
Western blot analysis of SEPHS2 expression in K-562 whole cell lysate.
Application
Western blot analysis of SEPHS2 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.
Application
Western blot analysis of SEPHS2 expression in non-transfected 293T: (A), human SEPHS2 transfected 293T: (B) and K-562 (C) whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
