Application
Western blot analysis of PEPCK-M expression in non-transfected: (A) and mouse PEPCK-M transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PEPCK-M expression in ZR-75-1 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | PEPCK-M Antibody (C-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PEPCK-M of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Normal adjustment to changes in blood glucose levels depends on Insulin signaling as well as enzymes involved in the regulation of gluconeogenesis. Pathological changes to this process are central to the type 2 diabetes phenotype. Phosphoenolpyruvate carboxykinase (PEPCK) plays an important role in this process by stimulating hepatic glucose production. PEPCK expression increases in response to glucagon and glucocorticoids, while Insulin suppresses expression. Modulation of the signals governing PEPCK levels present a potential therapeutic approach to the treatment of Insulin resistance and consequently obesity. The cytosolic form of PEPCK, known as PEPCK-C, and the mitochondrial form, known as PEPCK-M, are encoded by two different nuclear genes in mouse, human and chicken. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PEPCK-M of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PEPCK-M of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 5106 |
|---|
Application
Western blot analysis of PEPCK-M expression in non-transfected: (A) and mouse PEPCK-M transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PEPCK-M expression in ZR-75-1 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
