Application
Western blot analysis of ZNF545 expression in non-transfected: (A) and human ZNF545 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of ZNF545 expression in transfected 293T whole cell lysate.| Product Name | ZNF545 Antibody (S-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against ZNF545 of human origin |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain Krüppel-type DNA binding domains that are frequently observed to be involved in sequence-specific DNA binding. ZNF545 is a 532 amino acid transcriptional regulator belonging to the Krüppel C2H2-type zinc-finger protein family. ZNF545 localizes to the nucleus and contains 13 C2H2-type zinc fingers and a KRAB domain. Two isoforms of ZNF545 are formed due to alternative splicing. ZNF545 is encoded by a gene located on chromosome 19, which contains a diversity of interesting genes and is recognized for having the greatest gene density of the human chromosomes. chromosome 19 is the genetic home for a number of immunoglobulin superfamily members, including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG family and Fc receptors (FcR). Key genes for eye color and hair color also map to chromosome 19. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| recommended for detection of ZNF545 of mouse, rat and human origin by WB, IP and ELISA: WB:1:50~1:100 IP:1:10~1:50 ELISA:1:500~2500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against ZNF545 of human origin |
|---|---|
| Tissue Specificity | raised against ZNF545 of human origin |
| Entrez Gene | 284406 |
|---|
Application
Western blot analysis of ZNF545 expression in non-transfected: (A) and human ZNF545 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of ZNF545 expression in transfected 293T whole cell lysate.| Application Notes | recommended for detection of ZNF545 of mouse, rat and human origin by WB, IP and ELISA: WB:1:50~1:100 IP:1:10~1:50 ELISA:1:500~2500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
