Application
Western blot analysis of HRI expression in non-transfected 293T: (A), mouse HRI transfected 293T: (B) and HEL 92.1.7 (C) whole cell lysates.Application
Western blot analysis of HRI expression in non-transfected 293T: (A), mouse HRI transfected 293T: (B) and HEL 92.1.7 (C) whole cell lysates.| Product Name | HRI Antibody (H-165) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 27-165 mapping near the N-terminus of HRI of human origin |
| Modification Notes | HRI (heme-regulated inhibitor kinase) phosphorylates the alpha subunit of eIF2alpha kinase, which plays an important role in translational regulation during heme deficiency. HRI is activated in response to a number of environmental conditions, including heme deficiency, heat shock, and oxidative stress. Autophosphorylation is essential for the activation of HRI, which causes an arrest of initiation of protein synthesis. Both HSP 90 and HSC 70 are necessary for all stress-induced HRI activation. Furthermore, HSC 70 is required for the folding and transformation of HRI into an active kinase and is subsequently required to negatively attenuate the activation of transformed HRI. Both the N-terminus and the kinase insertion domain, which are unique to HRI, are involved in the heme binding and the heme regulation of HRI. The human HRI gene maps to chromosome 7p22 and encodes a 630 amino acid protein expressed mainly in erythroid cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HRI of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 27-165 mapping near the N-terminus of HRI of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 27-165 mapping near the N-terminus of HRI of human origin |
| Entrez Gene | 27102 |
|---|
Application
Western blot analysis of HRI expression in non-transfected 293T: (A), mouse HRI transfected 293T: (B) and HEL 92.1.7 (C) whole cell lysates.
Application
Western blot analysis of HRI expression in non-transfected 293T: (A), mouse HRI transfected 293T: (B) and HEL 92.1.7 (C) whole cell lysates.| Application Notes | recommended for detection of HRI of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
