Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.Application
Western blot analysis of ETAR expression in rat testis tissue extract.Application
Western blot analysis of ETAR expression in non-transfected: (A) and human ETAR transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ETAR expression in mouse heart (A) and mouse lung (B) tissue extracts.| Product Name | ETAR Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-80 mapping within an N-terminal extracellular domain of ETAR of human origin |
| Modification Notes | Endothelin receptor A (ETAR), also known as EDNRA, ET1 Receptor, ETA, EDN1 and ET-AR is a member of the Guanine-binding regulatory protein-coupled receptor family. ETAR binds endothelins and has the highest affinity for its ligand ET1, as compared to the ETBR receptor. Both ET receptors, ETAR and ETBR, are activated by ET1, which results in inhibition of active lens sodium-potassium transport. Activation of the ET receptors also causes an increase in cytoplasmic calcium concentration in cultured lens epithelial cells. In addition, ETAR induces arachidonic acid accumulation. ETAR has seven hydrophobic transmembrane domains and is expressed in aorta, lung, atrium, kidney, placenta and prostate. Specifically, placental vascular smooth muscle cells (PVSMCs) exclusively express ETAR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of all ETAR isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-80 mapping within an N-terminal extracellular domain of ETAR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-80 mapping within an N-terminal extracellular domain of ETAR of human origin |
| Entrez Gene | 1909 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.
Application
Western blot analysis of ETAR expression in rat testis tissue extract.
Application
Western blot analysis of ETAR expression in non-transfected: (A) and human ETAR transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ETAR expression in mouse heart (A) and mouse lung (B) tissue extracts.| Application Notes | recommended for detection of all ETAR isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
