Application
Western blot analysis of PCDHB15 expression in non-transfected: (A) and human PCDHB15 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of PCDHB15 expression in non-transfected: (A) and human PCDHB15 transfected: (B) 293T whole cell lysates.| Product Name | PCDHB15/Pcdhb22 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping within an N-terminal extracellular domain of PCDHB15 of human origin |
| Modification Notes | Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. Pcdhb22 (protocadherin beta 22), also known as PcdhbV or Pcdhb15, is a 794 amino acid murine protein that is thought to function as a calcium-dependent cell-adhesion protein. Pcdhb22 is a homolog of human PCDHB15 (protocadherin beta 15), a 787 amino acid protein that is one of 16 proteins in the protocadherin beta cluster. Unlike the alpha and gamma gene clusters whose genes are spliced to downstream constant region exons during transcription, members of the beta cluster (such as PCDHB15) do not use constant-region exons to produce mRNAs. As a result, each protocadherin beta gene encodes the transmembrane, extracellular and short cytoplasmic domains of the protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PCDHB15 of human origin and Pcdhb22 of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping within an N-terminal extracellular domain of PCDHB15 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping within an N-terminal extracellular domain of PCDHB15 of human origin |
| Entrez Gene | 56121 |
|---|
Application
Western blot analysis of PCDHB15 expression in non-transfected: (A) and human PCDHB15 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of PCDHB15 expression in non-transfected: (A) and human PCDHB15 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PCDHB15 of human origin and Pcdhb22 of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
