Application
Western blot analysis of EPI64 expression in non-transfected: (A) and mouse EPI64 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of EPI64 expression in non-transfected: (A) and human EPI64 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of EPI64 expression in mouse embryo tissue extract.| Product Name | EPI64 Antibody (H-128) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 381-508 mapping at the C-terminus of EPI64 of human origin |
| Modification Notes | The Na+/H+ exchange protein (NHE) functions in transepithelial Na+ absorption and is primarily expressed in the intestinal and renal brush border membrane. NHE regulatory factor 1 (NHERF-1) interacts with NHE through two PDZ (for PSD-95, Discs-large, and ZO-1 homology) domains, which are protein-protein interaction modules that associate with specific C-terminal motifs on target proteins. Also known as EBP50, NHERF-1 facilitates cAMP inhibition of NHE to decrease Na+ adsorption. NHERF-2, also known as E3KARP, is ubiquitously expressed as a protein which also functions in NHE2 regulation. EBP-PDZ interactor (EPI64) contains a C-terminal -DTYL sequence that binds to the first PDZ domain of NHERF-1 and NHERF-2. EPI64 is ubiquitously expressed and localizes with NHERF-1 in vitro. The gene encoding human EPI64 maps to chromosome 22q12.1-qter. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of EPI64 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 381-508 mapping at the C-terminus of EPI64 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 381-508 mapping at the C-terminus of EPI64 of human origin |
| Entrez Gene | 83874 |
|---|
Application
Western blot analysis of EPI64 expression in non-transfected: (A) and mouse EPI64 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of EPI64 expression in non-transfected: (A) and human EPI64 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of EPI64 expression in mouse embryo tissue extract.| Application Notes | recommended for detection of EPI64 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
