Application
Western blot analysis of CARP expression in Hep G2 nuclear extract (A) and human heart tissue extract (B).Application
Western blot analysis of CARP expression in Sol8 nuclear extract (A), P 23 whole cell lysate (B) and mouse heart (C) and rat heart (D) tissue extracts.Application
Western blot analysis of CARP expression in mouse heart tissue extract.Application
Western blot analysis of CARP expression in non-transfected 293T: (A), human CARP transfected 293T: (B) and Caki-1 (C) whole cell lysates.Application
Western blot analysis of CARP expression in non-transfected 293T: (A), human CARP transfected 293T: (B) and Caki-1 (C) whole cell lysates.Application
Western blot analysis of CARP expression in non-transfected 293T: (A), mouse CARP transfected 293T: (B) and Caki-1 (C) whole cell lysates.| Product Name | CARP Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-120 mapping at the N-terminus of CARP of human origin |
| Modification Notes | A proposed genetic marker of cardiac hypertrophy, CARP (cardiac ankyrin repeat protein) is a nuclear protein with an established role in regulation of cardiac gene expression. A distinct increase in CARP expression occurs in rats with abdominal aorta constriction, spontaneous hypertension and Dahl salt-sensitivity. In cardiomyocytes, CARP inhibits transcription of both cardiac troponin C and atrial natriuretic factor. Specifically, expression of the CARP gene, which lies downstream of the cardiac homeobox gene Nkx2.5, inhibits Nkx2.5 transactivation of atrial natriuretic factor promoter. An increase in CARP expression is observed in the ventricular tissue of patients with end-stage heart failure. The major Ca2+ binding protein of cardiac sarcoplasmic reticulum (SR), Calsequestrin (CSQ), upregulates the CARP gene, and may contribute to the development of cardiac hypertrophy and fibrosis. TGF∫ induces CARP expression in vascular smooth muscle cells (VSMCs), wherein CARP may mediate the inhibitory effects of TGF∫ on VSMC proliferation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CARP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-120 mapping at the N-terminus of CARP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-120 mapping at the N-terminus of CARP of human origin |
| Entrez Gene | 27063 |
|---|
Application
Western blot analysis of CARP expression in Hep G2 nuclear extract (A) and human heart tissue extract (B).
Application
Western blot analysis of CARP expression in Sol8 nuclear extract (A), P 23 whole cell lysate (B) and mouse heart (C) and rat heart (D) tissue extracts.
Application
Western blot analysis of CARP expression in mouse heart tissue extract.
Application
Western blot analysis of CARP expression in non-transfected 293T: (A), human CARP transfected 293T: (B) and Caki-1 (C) whole cell lysates.
Application
Western blot analysis of CARP expression in non-transfected 293T: (A), human CARP transfected 293T: (B) and Caki-1 (C) whole cell lysates.
Application
Western blot analysis of CARP expression in non-transfected 293T: (A), mouse CARP transfected 293T: (B) and Caki-1 (C) whole cell lysates.| Application Notes | recommended for detection of CARP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
