Application
Western blot analysis of CD3-δ expression in CCRF-CEM whole cell lysate.Application
Western blot analysis of CD3-δ expression in CCRF-CEM (A) and HuT 78 (B) whole cell lysates.| Product Name | CD3-δ Antibody (FL-171) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-171 representing full length CD3-δ of human origin |
| Modification Notes | The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (©é), a heterodimer of delta and epsilon chains (∂é) and a homodimer of two zeta chains (ΩΩ) or a heterodimer of zeta and eta chains (Ωh). The Ω and h chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The ©, é and ∂ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the Ω chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the Ω chain ITAMs has been solved. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD3-δ and, to a lesser extent, CD3-γ of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-171 representing full length CD3-&delta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-171 representing full length CD3-δ of human origin |
| Entrez Gene | 915 |
|---|
Application
Western blot analysis of CD3-δ expression in CCRF-CEM whole cell lysate.
Application
Western blot analysis of CD3-δ expression in CCRF-CEM (A) and HuT 78 (B) whole cell lysates.| Application Notes | recommended for detection of CD3-δ and, to a lesser extent, CD3-γ of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
