Application
Western blot analysis of ANKH expression in non-transfected: (A) and human ANKH transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ANKH expression in non-transfected: (A) and mouse ANKH transfected: (B) 293T whole cell lysates and rat skeletal muscle tissue extract (C).| Product Name | ANKH Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ANKH of human origin |
| Modification Notes | ANKH (progressive ankylosis protein homolog) is a multi-pass transmembrane protein involved in bone mineralization and joint maintenance. ANKH is a ubiquitously expressed protein with high expression levels in the embryonic developing articular cartilage of joints in the elbow, wrist, shoulders and digits. Expression of ANKH is regulated by cytokines and various growth factors. ANKH functions as a transporter, regulating the extracellular transport of inorganic pyrophosphates (PPi). The extracellular level of inorganic pyrophosphates can both inhibit and promote different types of pathologic mineralization. Mutations in ANKH are associated with various human skeletal defects including calcium pyrophosphate deposition disease (CPPD) and craniometaphyseal dysplasia Jackson type (CMDJ). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ANKH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ANKH of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ANKH of human origin |
| Entrez Gene | 56172 |
|---|
Application
Western blot analysis of ANKH expression in non-transfected: (A) and human ANKH transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ANKH expression in non-transfected: (A) and mouse ANKH transfected: (B) 293T whole cell lysates and rat skeletal muscle tissue extract (C).| Application Notes | recommended for detection of ANKH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
