| Product Name | p-LAT Antibody (Tyr 175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 175 of LAT of mouse origin |
| Modification | Phosphoralated |
| Modification Notes | LAT (linker of activation of T cells), is an integral membrane protein which links the proximal and distal portions of the TCR signaling pathway. LAT is atransmembrane protein that becomes rapidly tyrosine-phosphorylated after TCR engagement. Phosphorylation of LAT creates binding sites for the Src homology 2 (SH2) domains of other proteins, including PLC-gamma1, Grb2, Gads, Grap, 3BP2, and Shb, and indirectly binds SOS, c-Cbl, Vav, SLP-76, and Itk. T-cell-receptor-mediated LAT phosphorylation is critical for the membrane recruitment of signalling complexes required for T-cell activation. Tyr 191 and Tyr 171 phosphorylation are required for T-cell activation. Tyr 171 and Tyr 191 phosphorylation is also necessary for Grb2 and Gads binding, and are necessary and sufficient to achieve a Ca(2+) flux following TCR stimulation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of Tyr 175 phosphorylated LAT of mouse and rat origin by WB, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 175 of LAT of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 175 of LAT of mouse origin |
| Entrez Gene | 16797 |
|---|
| Application Notes | recommended for detection of Tyr 175 phosphorylated LAT of mouse and rat origin by WB, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
