Application
Western blot analysis of v-SNARE Ykt6p expression in HeLa whole cell lysate.| Product Name | v-SNARE Ykt6p Antibody (FL-198) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-198 representing full length v-SNARE Ykt6p of human origin |
| Modification Notes | Membrane traffic in eukaryotic cells requires the interaction of a vesicle-associated soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein receptor (v-SNARE) on transport vesicles with a SNARE on the target membrane (t-SNARE) (1). Both v- and t-SNAREs are compartment-specific and bind each other directly and specifically (2). The v-SNAREs Ykt6p and Vti1p are involved in ER-Golgi and intra-Golgi membrane trafficking (1,3). For v-SNARE Ykt6p, membrane interaction is mediated through a cysteine/aliphatic/aliphatic/methionine or histidine (CAAX) C-terminal motif, a consensus sequence involved in prenylated membrane anchoring (4). The v-SNARE Vti1p interacts with the prevacuolar t-SNARE Pep12p in Golgi prevacuolar transport and with the cis-Golgi t-SNARE Sed5p in traffic to the cis-Golgi (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of v-SNARE Ykt6p of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-198 representing full length v-SNARE Ykt6p of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-198 representing full length v-SNARE Ykt6p of human origin |
| Entrez Gene | 10652 |
|---|
Application
Western blot analysis of v-SNARE Ykt6p expression in HeLa whole cell lysate.| Application Notes | recommended for detection of v-SNARE Ykt6p of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
