Application
Western blot analysis of ARA70 expression in non-transfected: (A) and human ARA70 transfected: (B) 293T whole cell lysates.| Product Name | ARA70 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ARA70 of human origin |
| Modification Notes | Androgen receptor (AR) coactivator ARA70, also designated RFG and ELE1, is a putative co-activator that specifically enhances the activity of the androgen receptor. In human thyroid carcinomas, the Ret proto-oncogene fuses to ARA70 to form Ret/PTC3 by an intrachromosomal inversion of chromosome 10 in vivo. ARA70 is expressed as two isoforms, ARA70å and ARA70∫. The shorter variant, ARA70∫, results from an internal 985-bp deletion. ARA70å is widely expressed, and its expression is highest in testis and adipose tissues, whereas ARA70∫ is solely expressed in the testis. ARA70å can function as a ligand-enhanced co-activator of PPAR© in adipocytes. However, PPAR©-ARA70 transactivation can be squelched by AR, which suggests cross talk between PPAR©- and AR-mediated response. ARA70å has no intrinsic transcription activation domain or histone acetyltransferase activity, but it interacts with histone acetyltransferase, p/CAF, CBP and p300/CBP-associated factors, and the basal transcription factor TFIIB. The interaction between ARA70 and AR occurs through the ligand-binding domain. The presence of ARA70 can enhance the androgenic activity of 17-b Estradiol (E2) and antiandrogens toward AR. ARA70 may be involved in prostate carcinogenesis and ovarian cancer and may serve as a key mediator of estrogen-androgen synergism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of ARA70 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ARA70 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ARA70 of human origin |
| Entrez Gene | 8031 |
|---|
Application
Western blot analysis of ARA70 expression in non-transfected: (A) and human ARA70 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ARA70 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
