Application
Western blot analysis of GluR-5/6/7 expression in 293T whole cell lysate and mouse brain tissue extract.Application
Western blot analysis of GluR-5/6/7 expression in EOC 20 whole cell lysate (A) and rat brain tissue extract (B).| Product Name | GluR-5/6/7 Antibody (M-270) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 31-300 mapping within an extracellular domain of GluR-5 of mouse origin |
| Modification Notes | Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors are co-localized with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to -7. The kainate/AMPA receptors are primarily responsible for the fast excitatory neuro-transmission by glutamate, whereas the NMDA receptors are functionally characterized by a slow kinetic and a high permeability for Ca2+ ions. The NMDA receptors consist of five subunits: epsilion 1, 2, 3, 4 and one zeta subunit. The zeta subunit is expressed throughout the brainstem, whereas the four epsilon subunits display limited distribution. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GluR-5, 6 and 7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 31-300 mapping within an extracellular domain of GluR-5 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 31-300 mapping within an extracellular domain of GluR-5 of mouse origin |
| Entrez Gene | 2897 |
|---|
Application
Western blot analysis of GluR-5/6/7 expression in 293T whole cell lysate and mouse brain tissue extract.
Application
Western blot analysis of GluR-5/6/7 expression in EOC 20 whole cell lysate (A) and rat brain tissue extract (B).| Application Notes | recommended for detection of GluR-5, 6 and 7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
