Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Dyrk1A expression in SH-SY5Y whole cell lysate.Application
Western blot analysis of Dyrk1A expression in PC-12 whole cell lysate.Application
Western blot analysis of Dyrk1A expression in HL-60 (A) and SH-SY5Y (B) whole cell lysates.| Product Name | Dyrk1A Antibody (H-143) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 621-763 mapping at the C-terminus of Dyrk1A of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Dyrk (for dual specificity tyrosine phosphorylation regulated kinase) is the homolog of the Drosophila mnb (minibrain) gene which is required for neurogenesis. Dyrk is a dual-specificity tyrosine kinase and serine/threonine kinase, which is itself regulated by tyrosine phosphorylation. Several mammalian Dyrk related proteins have been identified and are thought to compose a family of dual specificity protein kinases. Dyrk family members, including Dyrk1A (dual specificity tyrosine-phosphorylation-regulated kinase 1A), Dyrk1B, Dyrk1C, Dyrk2, Dyrk3, Dyrk4A and Dyrk4B, are thought to be involved in diverse cellular functions. Localized to the nucleus and highly expressed in testis, muscle and the developing nervous system, Dyrk1A, also known as MNB or MNBH, functions to phosphorylate serine, threonine and tyrosine residues on various substrates involved in signaling pathways that regulate cell proliferation. Dyrk1A is a candidate gene for learning defects that are involved in Downs syndrome (DS), suggesting a possible role for Dyrk1A in the development of DS. Four isoforms of Dyrk1A exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | equine, canine, bovine, porcine and avian |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 621-763 mapping at the C-terminus of Dyrk1A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 621-763 mapping at the C-terminus of Dyrk1A of human origin |
| Entrez Gene | 1859 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Dyrk1A expression in SH-SY5Y whole cell lysate.
Application
Western blot analysis of Dyrk1A expression in PC-12 whole cell lysate.
Application
Western blot analysis of Dyrk1A expression in HL-60 (A) and SH-SY5Y (B) whole cell lysates.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
