Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing nuclear and cytoplasmic staining of glandular cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of BAP1 expression in A-431 (A), HeLa (B) and SW480 (C) whole cell lysates.| Product Name | BAP1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 430-729 mapping at the C-terminus of BAP1 of human origin |
| Modification Notes | Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is a ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of BAP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 430-729 mapping at the C-terminus of BAP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 430-729 mapping at the C-terminus of BAP1 of human origin |
| Entrez Gene | 8314 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing nuclear and cytoplasmic staining of glandular cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of BAP1 expression in A-431 (A), HeLa (B) and SW480 (C) whole cell lysates.| Application Notes | recommended for detection of BAP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
