Application
Western blot analysis of RAP1 expression in non-transfected: (A) and mouse RAP1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RAP1 expression in non-transfected: (A) and human RAP1 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of lymphoid cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of RAP1 expression in HeLa (A), HL-60 (B), IMR-32 (C), C32 (D) and A-431 (E) nuclear extracts and HT-1080 whole cell lysate (F).| Product Name | RAP1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 153-300 mapping within an internal region of RAP1 of human origin |
| Modification Notes | Telomeres are DNA-protein structures that protect the ends of linear chromosomes and help maintain genomic stability and cell phenotype. Mammalian telomeric proteins consist of TRF1 (telomeric repeat binding factor), TRF2, tankyrase and TIN2, which have no recognized orthologs in the budding yeast, Saccharomyces cerevisiae, and RAP1, which is an ortholog to the yeast telomeric protein scRap1. Like scRap1, mammalian RAP1 regulates telomere elongation. RAP1 interacts with two proteins, Rif1 and Rif2, which contribute to telomere length homeostasis. Unlike scRap1, which binds telomeric DNA directly, RAP1 is recruited to telomeres by TRF2. The functional and structural similarities of scRap1 to mammalian RAP1 suggest that the budding yeast preserved RAP1 at telomeres, but lost the TRF component. The telomeric protein TRF1 requires TIN2 to control telomere length in human cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of RAP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 153-300 mapping within an internal region of RAP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 153-300 mapping within an internal region of RAP1 of human origin |
| Entrez Gene | 54386 |
|---|
Application
Western blot analysis of RAP1 expression in non-transfected: (A) and mouse RAP1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RAP1 expression in non-transfected: (A) and human RAP1 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of lymphoid cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of RAP1 expression in HeLa (A), HL-60 (B), IMR-32 (C), C32 (D) and A-431 (E) nuclear extracts and HT-1080 whole cell lysate (F).| Application Notes | recommended for detection of RAP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
