Application
Western blot analysis of Profilin-1 expression in non-transfected: (A) and human Profilin-1 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Profilin-1 expression in non-transfected: (A) and mouse Profilin-1 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of Profilin-1/2 expression in human platelet (A), Hep G2 (B) and JAR (C) whole cell lysates.| Product Name | Profilin-1/2 Antibody (FL-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-140 representing full length Profilin-1 of human origin |
| Modification Notes | Profilins regulate Actin polymerization by binding to and sequestering the Actin monomer. Profilins act as a nucleotide exchange factor that charges actin with ATP after binding the Actin monomer through a 1:1 stoichiometric relationship. Human Profilin-1 and Profilin-2 are encoded by two separate genes mapping to chromosomes 17p13.3 and 3q25.1-q25.2, respectively. Both Profilin-1 and Profilin-2 are abundantly expressed in kidney. Profilin-1 is highly expressed in lung, liver, placenta and kidney while profilin-2 is highly expressed in brain and skeletal muscle. In axonal and dendritic processes of mouse brain, Profilins co-localize with Dyamin I and Synapsin. Profilin may play a role in mediating cell adhesion. The overexpression of Profilin in endo-thelial cells results in increased adhesion to Fibronectin. In food allergy, plant Profilin is considered a pan-allergen. Case studies indicate individuals with allergies to various foods including celery, carrots, zucchini and pea-nuts are actually sensitive to the Profilin proteins in these foods. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Profilin-1, and, to a lesser extent, Profilin-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-140 representing full length Profilin-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-140 representing full length Profilin-1 of human origin |
| Entrez Gene | 5216 |
|---|
Application
Western blot analysis of Profilin-1 expression in non-transfected: (A) and human Profilin-1 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Profilin-1 expression in non-transfected: (A) and mouse Profilin-1 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of Profilin-1/2 expression in human platelet (A), Hep G2 (B) and JAR (C) whole cell lysates.| Application Notes | recommended for detection of Profilin-1, and, to a lesser extent, Profilin-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
