Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing cytoplasmic and membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse eye frozen section showing basement membrane and stroma staining.| Product Name | Perlecan Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 4092-4391 mapping at the C-terminus of Perlecan of human origin |
| Modification Notes | Perlecan is part of a large family of heparan sulfate proteoglycans (HSPGs). As key components of cell surfaces and extracellular matrices, HSPGs modulate growth factor activities and thereby influence cell growth and differentiation. Additionally, HSPGs play a critical role in regulating tumor cell metastasis by mediating cell adhesion and the activities of growth and angiogenic factors. Perlecan consists of five distinct structural domains that interact with a number of matrix molecules, cytokines and growth factors to influence cartilage development and neuromuscular junction activity. Antithrombin, a key regulator of blood coagulation proteases, and TGFb1 act as inhibitors and stimulators of Perlecan expression, respectively, interactions which may provide avenues for therapeutic intervention in certain types of cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Perlecan of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 4092-4391 mapping at the C-terminus of Perlecan of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 4092-4391 mapping at the C-terminus of Perlecan of human origin |
| Entrez Gene | 3339 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing cytoplasmic and membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse eye frozen section showing basement membrane and stroma staining.| Application Notes | recommended for detection of Perlecan of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
