Application
Western blot analysis of GTBP expression in HeLa whole cell lysate.Application
Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic staining.Application
Western blot analysis of GTBP expression in HeLa (A), SW480 (B) and DU 145 (C) nuclear extracts.| Product Name | GTBP Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1221-1360 mapping at the C-terminus of GTBP of human origin |
| Modification Notes | The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GTBP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1221-1360 mapping at the C-terminus of GTBP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1221-1360 mapping at the C-terminus of GTBP of human origin |
| Entrez Gene | 2956 |
|---|
Application
Western blot analysis of GTBP expression in HeLa whole cell lysate.
Application
Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic staining.
Application
Western blot analysis of GTBP expression in HeLa (A), SW480 (B) and DU 145 (C) nuclear extracts.| Application Notes | recommended for detection of GTBP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
