Application
Western blot analysis of CapG expression in HL-60 whole cell lysate.Application
Western blot analysis of CapG expression in non-transfected 293T: (A), human CapG transfected 293T: (B) and U-937 (C) whole cell lysates.Application
Western blot analysis of CapG expression in non-transfected 293T: (A), mouse CapG transfected 293T: (B) and HL-60 (C) whole cell lysates.Application
Western blot analysis of CapG expression in non-transfected: (A) and mouse CapG transfected: (B) 293T whole cell lysates.| Product Name | CapG Antibody (H-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 256-340 mapping at the C-terminus of CapG of human origin |
| Modification Notes | Caldesmon, Filamin 1, Nebulin, Plastin, ADF, Gelsolin, CapG, Dematin and Cofilin are differentially expressed Actin-binding proteins. Both muscular (CDh) and non-muscular (CD1) forms of Caldesmon bind to Actin as well as to Calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CD1 is widely expressed in non-muscle tissues and cells. CapG, also designated Actin-regulatory protein and macrophage-capping protein, is a macrophage-specific protein that reversibly blocks the barbed ends of Actin filaments, but does not sever preformed ones. The interactions of CapG with Actin may be important in the regulation of nuclear and cytoplasmic structures. CapG is a calcium-sensitive DNA-binding protein that plays a role in macrophage function. It is expressed in macrophages and macrophage-like cells and can localize both to the nucleus and the cytoplasm. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CapG of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 256-340 mapping at the C-terminus of CapG of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 256-340 mapping at the C-terminus of CapG of human origin |
| Entrez Gene | 822 |
|---|
Application
Western blot analysis of CapG expression in HL-60 whole cell lysate.
Application
Western blot analysis of CapG expression in non-transfected 293T: (A), human CapG transfected 293T: (B) and U-937 (C) whole cell lysates.
Application
Western blot analysis of CapG expression in non-transfected 293T: (A), mouse CapG transfected 293T: (B) and HL-60 (C) whole cell lysates.
Application
Western blot analysis of CapG expression in non-transfected: (A) and mouse CapG transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CapG of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
