Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of gp130 expression in WEHI-231 (A) and PC-12 (B) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.Application
Western blot analysis of gp130 expression in BW5147 whole cell lysate.Application
Western blot analysis of human recombinant gp130.| Product Name | gp130 Antibody (H-255) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 365-619 mapping within the extracellular domain of gp130 of human origin |
| Modification Notes | IL-6 activates intracellular signaling through binding a receptor consisting of a ligand-binding protein (IL-6R) and a second protein. IL-6 first binds to IL-6R which subsequently associates with a gp130 dimer. The active signaling complex consists of at minimum IL-6, IL-6R and a dimer of two gp130 proteins that are linked by a disulfide bond. A soluble form of IL-6R is generated by proteolytic cleavage of the membrane-bound precursor and can function as an agonistic molecule that can actively participate in cell-to-cell signaling. The second subunit of the IL-6 complex, gp130, also functions as a component of several additional receptor complexes including leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and IL-11. LIF binds to the LIF receptor with low affinity and to a complex of the LIF receptor and gp130 with high affinity while OSM appears to bind to gp130 with low affinity and to a complex of gp130 and the LIF receptor with high affinity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of gp130 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 365-619 mapping within the extracellular domain of gp130 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 365-619 mapping within the extracellular domain of gp130 of human origin |
| Entrez Gene | 3572 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of gp130 expression in WEHI-231 (A) and PC-12 (B) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.
Application
Western blot analysis of gp130 expression in BW5147 whole cell lysate.
Application
Western blot analysis of human recombinant gp130.| Application Notes | recommended for detection of gp130 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
