Application
Western blot analysis of Munc13-4 expression in non-transfected: (A) and human Munc13-4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Munc13-4 expression in 293T (A) and HeLa (B) whole cell lysates.| Product Name | Munc13-4 Antibody (H-150) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 136-285 mapping near the N-terminus of Munc13-4 of human origin |
| Modification Notes | Munc13-4 is a member of the Munc13 family and is a homolog to Munc13-1. Munc13-4 lacks the C1 domain and N-terminal extension that are present in other Munc13 family members. It is a peripheral membrane, GTP-Rab 27a-binding protein. Munc13-4 has an ubiquitous tissue distribution, however, unlike related proteins Munc13-1, -2 and -3, Munc13-4 is mainly expressed outside the nervous system. High expression levels of Munc13-4 have been seen in mucous goblet and alveolar type II cells of the lung, as well as in cytotoxic T lymphocytes and mast cells. Munc13-4 localizes to secretory lysosomes. Overexpression of Munc13-4 enhances degranulation of mast cell secretory lysosomes, suggesting that it positively regulates secretory lysosome fusion and exocytosis. Mutations in Munc13-4 cause familial hemophagocytic lymphohistiocytosis subtype 3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Munc13-4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 136-285 mapping near the N-terminus of Munc13-4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 136-285 mapping near the N-terminus of Munc13-4 of human origin |
| Entrez Gene | 201294 |
|---|
Application
Western blot analysis of Munc13-4 expression in non-transfected: (A) and human Munc13-4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Munc13-4 expression in 293T (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of Munc13-4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
