Application
Western blot analysis of Calpain 7 expression in non-transfected 293T: (A), mouse Calpain 7 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Calpain 7 expression in HeLa (A), MCF7 (B) and DU 145 (C) nuclear extracts.| Product Name | Calpain 7 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Calpain 7 of human origin |
| Modification Notes | Calpains are calcium-activated thiol proteases. Calpain 7 (also known as PalBH) is a member of the Non-EF-hand subfamily of calpains and may be calcium independent. Calpain 7 has 813 amino acid residues and is a divergent member of the calpain family. It has only 26-35% shared identity to other members and most of this homology is in the protease domain. Calpain 7 seems to be related to PalB, an Aspergillus nidulans protease that is involved in alkaline ambient pH adaptation. A long N-terminal domain (N) and a PalB homologous domain (PBH) flank the calpain protease domain of calpain 7. Calpain 7 appears to have a ubiquitous tissue distribution but is highly expressed in the brain. It localizes to the cytoplasm and the nucleus, but its activated form is found only in the nucleus. Calpain 7 is an atypical calpain that lacks domain IV and cannot form a dimer with the 30 kDa regulatory subunit. Upregulation of Calpain 7 in striatal or cortical tissue of Huntington’s Disease knock-in mice suggests that this protein may be involved in the onset of the disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Calpain 7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Calpain 7 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Calpain 7 of human origin |
| Entrez Gene | 23473 |
|---|
Application
Western blot analysis of Calpain 7 expression in non-transfected 293T: (A), mouse Calpain 7 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Calpain 7 expression in HeLa (A), MCF7 (B) and DU 145 (C) nuclear extracts.| Application Notes | recommended for detection of Calpain 7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
