Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Ang expression in HeLa (A), HUV-EC-C (B), HEL 92.1.7 (C), K-562 (D) whole cell lysates.Application
Western blot analysis of angiopoietin expression in ECV304 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed ECV304 cells showing cytoplasmic staining.| Product Name | Ang Antibody (H-98) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 400-498 of angiopoietin-1 (Ang-1) of human origin |
| Modification Notes | Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. Ang-1 is glycosylated and has a fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is an angiogenic factor that mediates blood vessel maturation and may be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), is a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression is restricted to sites of vascular remodeling. Ang-1 and Ang-2 are expressed in human malignant glioma. Ang-3 is a secretory protein expressed in adult human adrenal gland, placenta, lung, cultured human umbilical vein endothelial cells, thyroid gland, heart and small intestine. It acts as an antagonist. Ang-4 is expressed in heart and acts as an agonist. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ang-1, Ang-2 and Ang-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 400-498 of angiopoietin-1 (Ang-1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 400-498 of angiopoietin-1 (Ang-1) of human origin |
| Entrez Gene | 284 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Ang expression in HeLa (A), HUV-EC-C (B), HEL 92.1.7 (C), K-562 (D) whole cell lysates.
Application
Western blot analysis of angiopoietin expression in ECV304 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed ECV304 cells showing cytoplasmic staining.| Application Notes | recommended for detection of Ang-1, Ang-2 and Ang-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
