Application
Western blot analysis of CD5 expression in non-transfected: (A) and human CD5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD5 expression in MOLT-4 (A), CCRF-CEM (B), Jurkat (C), Ramos (D) and Daudi (E) whole cell lysates.Application
Western blot analysis of CD5 expression in HuT 78 (A) and CCRF-CEM (B) whole cell lysates.| Product Name | CD5 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of CD5 of human origin |
| Modification Notes | CD5 (also designated Lyt-1) has been identified as a transmembrane glycoprotein that is expressed on 70% of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. Activation of T cells through the T cell receptor (TCR) results in tyrosine phosphorylation of CD5, and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation. CD5 associates with the TCR/ CD3-z chain and with the Src family kinase Lck p56. In vitro studies have shown a 10- to 15-fold increase in the kinase activity of Lck bound to CD5. The B cell antigen, CD72, serves as a receptor for CD5. The consequence of CD5 binding to its cognate receptor is still in question and likely plays a role in thymic selection. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD5 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of CD5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of CD5 of human origin |
| Entrez Gene | 921 |
|---|
Application
Western blot analysis of CD5 expression in non-transfected: (A) and human CD5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD5 expression in MOLT-4 (A), CCRF-CEM (B), Jurkat (C), Ramos (D) and Daudi (E) whole cell lysates.
Application
Western blot analysis of CD5 expression in HuT 78 (A) and CCRF-CEM (B) whole cell lysates.| Application Notes | recommended for detection of CD5 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
