Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells.Application
Western blot analysis of FAK expression in HeLa whole cell lysate.Application
Western blot analysis of FAK expression in non-transfected: (A) and human FAK transfected: (B) 293T whole cell lysates.Application
Blots were probed with FAK (C-20): (A), p-FAK (Ser 722)-R: preincubated with its cognate phosphorylated peptide (B) and p-FAK (Ser 722)-R: pre-incubated with its cognate non-phosphorylated peptide (C).Application
Antibodies tested include FAK (C-20): (A) and FAK (C-903): (B).Application
FAK (C-20): Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.Application
Western blot analysis of 20 ng of mouse recombinat FAK fusion protein (B) and an equal amount of an unrelated fusion protein (C). Antibodies tested include FAK (C-20)-G: (A) and FAK (C-20): (B,C).| Product Name | FAK Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of FAK of human origin |
| Modification Notes | Focal adhesion kinase was initially identified as a major substrate for the intrinsic protein tyrosine kinase activity of Src encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only those basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Thus, it has been postulated that FAK may have an important in vivo role in the reepithelialization of human wounds. FAK protein tyrosine kinase activity has also been shown to increase in cells stimulated to grow by use of mitogenic neuropeptides or neurotransmitters acting through G protein coupled receptors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FAK p125 and FRNK p41 of mouse, rat, human, avian, zebrafish and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of FAK of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of FAK of human origin |
| Entrez Gene | 5747 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells.
Application
Western blot analysis of FAK expression in HeLa whole cell lysate.
Application
Western blot analysis of FAK expression in non-transfected: (A) and human FAK transfected: (B) 293T whole cell lysates.
Application
Blots were probed with FAK (C-20): (A), p-FAK (Ser 722)-R: preincubated with its cognate phosphorylated peptide (B) and p-FAK (Ser 722)-R: pre-incubated with its cognate non-phosphorylated peptide (C).
Application
Antibodies tested include FAK (C-20): (A) and FAK (C-903): (B).
Application
FAK (C-20): Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.
Application
Western blot analysis of 20 ng of mouse recombinat FAK fusion protein (B) and an equal amount of an unrelated fusion protein (C). Antibodies tested include FAK (C-20)-G: (A) and FAK (C-20): (B,C).| Application Notes | recommended for detection of FAK p125 and FRNK p41 of mouse, rat, human, avian, zebrafish and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
