Application
Western blot analysis of GAP-43 expression in rat cerebellum (A), rat brain (B) and mouse brain (C) tissue extracts.Application
Western blot analysis of GAP-43 expression in non-transfected: (A) and human GAP-43 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of GAP-43 expression in mouse brain tissue extract.| Product Name | GAP-43 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-100 mapping at the N-terminus of GAP-43 of human origin |
| Modification Notes | GAP-43 (growth associated protein 43, B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1 is a crucial component for regenerative response in the nervous system. It is present at high levels in neuronal growth cones during development and axonal regeneration. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The neuron-specific Elav/Hu family member, HuD, interacts with and stabilizes GAP-43 mRNA in developing neurons and leads to increased levels of GAP-43 protein. Heterozygous GAP-43 knockout mice with GAP-43 levels reduced by one-half dipslay significant memory impairments in cued conditioning or on tests of nociceptive or auditory perception. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of axonal membrane protein GAP-43 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-100 mapping at the N-terminus of GAP-43 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-100 mapping at the N-terminus of GAP-43 of human origin |
| Entrez Gene | 2596 |
|---|
Application
Western blot analysis of GAP-43 expression in rat cerebellum (A), rat brain (B) and mouse brain (C) tissue extracts.
Application
Western blot analysis of GAP-43 expression in non-transfected: (A) and human GAP-43 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of GAP-43 expression in mouse brain tissue extract.| Application Notes | recommended for detection of axonal membrane protein GAP-43 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
