Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of glycosylated SR-1A expression in Saos-2 (A), Daudi (B) and Raji (C) whole cell lysates.| Product Name | SR-1A Antibody (H-119) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 218-336 of 5-hydroxytryptamine (serotonin) receptor 1A of human origin |
| Modification Notes | The members of the G-protein-coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. b-adrenergic bound to adrenaline activates adenylyl cyclase, while a2-adrenergic receptor bound to adrenaline inhibits adenylyl cyclase. Like the a2-adrenergic receptor, serotonin receptor functions are also mediated by G proteins that inhibit the activity of adenylyl cyclase. The serotonin receptors have been classified into several categories, designated SR-1–7 (5HT1–7). Subtypes within the SR-1 group include SR-1A, -1B, -1D, -1E and -1F. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of serotonin 1A receptor (5-HT1A) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 218-336 of 5-hydroxytryptamine (serotonin) receptor 1A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 218-336 of 5-hydroxytryptamine (serotonin) receptor 1A of human origin |
| Entrez Gene | 3350 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of glycosylated SR-1A expression in Saos-2 (A), Daudi (B) and Raji (C) whole cell lysates.| Application Notes | recommended for detection of serotonin 1A receptor (5-HT1A) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
