Application
Western blot analysis of karyopherin β3 expression in non-transfected 293T: (A), human karyopherin β3 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervical cancer tissue showing cytoplasmic and nucleolar staining of tumor cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
karyopherin β3 (H-300): Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and perinuclear staining.Application
Western blot analysis of karyopherin β3 expression in HeLa (A), KNRK (B) and NIH/3T3 (C) whole cell lysates.| Product Name | karyopherin β3 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of karyopherin β3 of human origin |
| Modification Notes | Protein transport across the nucleus is a selective, multi-step process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Two cytosolic factors centrally involved in the recognition and docking process are the karyopherin å1 and karyopherin ∫1 subunits. Karyopherin å1 functions in the recognition and targeting of substrates destined for nuclear import, while karyopherin ∫1 serves as an adapter, tethering the karyopherin å1/substrate complex to docking proteins on the nuclear envelope, termed nucleoporins. Karyopherin å2 has been shown to complex with Epstein-Barr virus nuclear antigen 1 (EBNA-1). Certain RNA-binding proteins are imported to the nucleus by karyopherin ∫2, and karyopherin ∫3 appears to be involved in the import of some ribosomal proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of karyopherin β3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of karyopherin &beta 3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of karyopherin β3 of human origin |
| Entrez Gene | 3843 |
|---|
Application
Western blot analysis of karyopherin β3 expression in non-transfected 293T: (A), human karyopherin β3 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervical cancer tissue showing cytoplasmic and nucleolar staining of tumor cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
karyopherin β3 (H-300): Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and perinuclear staining.
Application
Western blot analysis of karyopherin β3 expression in HeLa (A), KNRK (B) and NIH/3T3 (C) whole cell lysates.| Application Notes | recommended for detection of karyopherin β3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
