Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of TRAF6 expression in HeLa whole cell lysate.Application
Western blot analysis of TRAF6 expression in mouse kidney extract.| Product Name | TRAF6 Antibody (H-274) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-274 mapping at the N-terminus of TRAF6 of human origin |
| Modification Notes | Tumor necrosis factor receptor-associated factor 6 (TRAF6) regulates adaptive immunity, innate immunity and bone metabolism. TRAF6 is a ubiquitin (Ub) ligase that mediates the activation of protein kinases, such as transforming growth factor ∫-activated kinase (TAK1) and I˚B kinase (IKK), by catalyzing the formation of a unique polyubiquitin chain linked through Lys 63 of Ub. TRAF6 is essential for activating NF˚B signaling pathway in response to interleukin-1 and Toll-like receptor ligands. The coiled-coil domain of TRAF6 is essential for its auto-ubiquitination and activating NF˚B signaling pathway. TRAF6 interacts with various protein kinases including IRAK1/IRAK, SRC and PKCΩ, which provides a link between distinct signaling pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TRAF6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-274 mapping at the N-terminus of TRAF6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-274 mapping at the N-terminus of TRAF6 of human origin |
| Entrez Gene | 7189 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of TRAF6 expression in HeLa whole cell lysate.
Application
Western blot analysis of TRAF6 expression in mouse kidney extract.| Application Notes | recommended for detection of TRAF6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
