Application
Western blot analysis of IL-1ra expression in A-431 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of human recombinant IL-1ra fusion protein.| Product Name | IL-1ra Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 26-135 mapping to an internal region of IL-1ra of human origin |
| Modification Notes | Two forms of interleukin-1, designated IL-1å and IL-1∫, have been described. Although encoded by distinct genes and exhibiting roughly only 25% sequence identity, IL-1å and IL-1∫ bind to the same receptor and seem to elicit similar biological responses. IL-1 production is generally thought to be associated with inflammation, but it has also been shown to be expressed during kidney development, thymocyte differentiation and cartilage degradation. IL-1 plays a critical role in the regulation of immune response and inflammation acting as an activator of T and B lymphocytes and natural killer (NK) cells. IL-1 receptor antagonist (IL-1ra) is a cytokine that inhibits IL-1å and IL-1∫ binding to interleukin receptors. By neutralizing the activity of IL-1, IL-1ra contributes to the inhibition of the immune and inflammatory responses and has been targeted as a drug for the treatment of severely active rheumatoid arthritis. There are four isoforms of IL-1ra that are produced as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IL-1ra of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 26-135 mapping to an internal region of IL-1ra of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 26-135 mapping to an internal region of IL-1ra of human origin |
| Entrez Gene | 3557 |
|---|
Application
Western blot analysis of IL-1ra expression in A-431 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic staining of squamous epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of human recombinant IL-1ra fusion protein.| Application Notes | recommended for detection of IL-1ra of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
