Application
Western blot analysis of PDGFR-β expression in non-transfected: (A) and human PDGFR-β transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PDGFR-β expression in CCD-1064Sk whole cell lysate.Application
Western blot analysis of PDGFR-β expression in non-transfected: (A) and human PDGFR-β transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells (A). Note membrane and cytoplasmic fluorescein immunostaining and nuclear DAPI counterstain. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue at high magnification (B) showing membrane and cytoplasmic localization.Application
Antibodies tested include PDGFR-β (P-20)-G: (A,B) and PDGFR-β (M-20): (C,D).| Product Name | PDGFR-β Antibody (P-20) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of PDGFR-β of human origin |
| Modification Notes | Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-å and -∫, which are endowed with protein tyrosine kinase domains. PDGFR-å can bind to both A and B subunits of PDGF, while PDGFR-∫ can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two å receptors, PDGF-AB induces dimerization of åå and å∫ and PDGF-BB induces the formation of three types of dimers, åå, å∫ and ∫∫. Translocation of the PDGFR-∫ gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PDGF receptor type β of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PDGFR-&beta of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PDGFR-β of human origin |
| Entrez Gene | 5159 |
|---|
Application
Western blot analysis of PDGFR-β expression in non-transfected: (A) and human PDGFR-β transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PDGFR-β expression in CCD-1064Sk whole cell lysate.
Application
Western blot analysis of PDGFR-β expression in non-transfected: (A) and human PDGFR-β transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells (A). Note membrane and cytoplasmic fluorescein immunostaining and nuclear DAPI counterstain. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue at high magnification (B) showing membrane and cytoplasmic localization.
Application
Antibodies tested include PDGFR-β (P-20)-G: (A,B) and PDGFR-β (M-20): (C,D).| Application Notes | recommended for detection of PDGF receptor type β of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
