Application
Western blot analysis of BMP-2 expression in non-transfected 293: (A), human BMP-2 transfected 293: (B) and THP-1 (C) whole cell lysates.Application
Western blot analysis of BMP-4 expression in non-transfected 293T: (A), mouse BMP-4 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | BMP-2/4 Antibody (H-51) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 300-350 mapping within an internal region of BMP-4 of human origin |
| Modification Notes | Tumor growth factor, or TGF∫, is the prototypic member of a family of secreted proteins that regulate cellular proliferation and differentiation. Related proteins include the activins and the bone morphogenic proteins or BMPs. Like TGF∫, the BMPs signal through a heteromeric receptor complex (TGF∫ R) composed of type I (TGF∫ RI) and type II (TGF∫ RII) receptors. Both the type I and the type II receptors contain an intrinsic serine/threonine kinase activity. Although signaling downstream of the TGF ∫ R is poorly understood, several proteins have been implicated. Six TGF∫/BMP effector proteins, designated Smad1-6, may function as tumor suppressors. Smad proteins have been suggested to be transcription factors, acting similarly to the Stat family which associates directly with activated receptors and then translocates to the nucleus. Evidence supporting this assertion is drawn from the observation that Smad3 physically associates with the TGF∫ R complex and that Smad1 is translocated to the nucleus 30-60 minutes after the addition of BMP-4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of BMP-2 and BMP-4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 300-350 mapping within an internal region of BMP-4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 300-350 mapping within an internal region of BMP-4 of human origin |
| Entrez Gene | 650 |
|---|
Application
Western blot analysis of BMP-2 expression in non-transfected 293: (A), human BMP-2 transfected 293: (B) and THP-1 (C) whole cell lysates.
Application
Western blot analysis of BMP-4 expression in non-transfected 293T: (A), mouse BMP-4 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of BMP-2 and BMP-4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
