Application
Western blot analysis under non-reducing conditions of VEGF expression in NIH/3T3 whole cell lysates (A,B).Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic and extracellular staining of hepatic arteries.Application
Antibodies tested include VEGF (147): (A) and VEGF (A-20): (B).| Product Name | VEGF Antibody (147) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-140 of VEGF of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-140 of VEGF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-140 of VEGF of human origin |
| Entrez Gene | 7422 |
|---|
Application
Western blot analysis under non-reducing conditions of VEGF expression in NIH/3T3 whole cell lysates (A,B).
Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic and extracellular staining of hepatic arteries.
Application
Antibodies tested include VEGF (147): (A) and VEGF (A-20): (B).| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
