Application
Western blot analysis of TTK expression in Ramos whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining in surface epithelial cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of TTK expression in HeLa (A), BJAB (B), HISM (C), C32 (D) and 3611-RF (E) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear staining.| Product Name | TTK Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of TTK of human origin |
| Modification Notes | Progression of cells through the cell cycle is regulated by variations in the levels and activities of a series of protein kinases as well as by oscillation in the levels of their regulatory subunits (i.e., cyclins). The full length sequence for a unique protein kinase of human origin, designated TTK, was cloned by screening a T cell expression library with anti-phosphotyrosine antibodies. Similarly, the mouse homolog of TTK was isolated from an embryonal carcinoma (EC) cell line by expression cloning. TTK/Esk are novel members of the serine-threonine/tyrosine family of protein kinases and are expressed in a broad range of proliferating human cells and tissues. TTK-Esk expression is reduced or absent in resting cells and in cells with a low proliferative index. When cells are induced to enter the cell cycle, levels of TTK mRNA, protein and kinase activity increase at the G1 to S phase of the cell cycle and peak in the G2 to M phase, suggesting that TTK/Esk may function as cell cycle regulatory components. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TTK of r and h origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of TTK of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of TTK of human origin |
| Entrez Gene | 7272 |
|---|
Application
Western blot analysis of TTK expression in Ramos whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining in surface epithelial cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of TTK expression in HeLa (A), BJAB (B), HISM (C), C32 (D) and 3611-RF (E) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear staining.| Application Notes | recommended for detection of TTK of r and h origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
