Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear and cytoplasmic staining of neuronal cells.Application
Western blot analysis of Skp1 p19 expression in mouse brain tissue extract.Application
Western blot analysis of Skp1 p19 expression in A-431 whole cell lysate.Application
Western blot analysis of Skp1 p19 expression in non-transfected: (A) and mouse Skp1 p19 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Skp1 p19 expression in non-transfected: (A) and human Skp1 p19 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.| Product Name | Skp1 p19 Antibody (H-163) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-163 representing full length Skp1 p19 of human origin |
| Modification Notes | The critical role that the family of regulatory proteins known as cyclins plays in eukaryotic cell cycle regulation is well established. The best characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of MPF (maturation promoting factor). Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F and retino-blastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Skp1 p19 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-163 representing full length Skp1 p19 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-163 representing full length Skp1 p19 of human origin |
| Entrez Gene | 6500 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear and cytoplasmic staining of neuronal cells.
Application
Western blot analysis of Skp1 p19 expression in mouse brain tissue extract.
Application
Western blot analysis of Skp1 p19 expression in A-431 whole cell lysate.
Application
Western blot analysis of Skp1 p19 expression in non-transfected: (A) and mouse Skp1 p19 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Skp1 p19 expression in non-transfected: (A) and human Skp1 p19 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.| Application Notes | recommended for detection of Skp1 p19 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
