Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules.Application
Western blot analysis of Rock-1 expression in Caki-1 (A), KNRK (B) and c4 (C) whole cell lysates and mouse liver tissue extract (D).Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.Application
Western blot analysis of Rock-1 expression in Hep G2 (A) and NIH/3T3 (B) whole cell lysates.| Product Name | Rock-1 Antibody (H-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 755-840 mapping within an internal region of Rock-1 of human origin |
| Modification Notes | Rho, the Ras-related small GTPase, is responsible for the regulation of Actin-based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described, including rhophilin, Rhotekin, citron and the serine/threonine kinase, protein kinase N. Two additional Rho-activated serine/threonine kinases have been described, designated Rock-1 and Rock-2 (also referred to as Roka, for Rho-associated coil-containing protein kinase). Rock-1 and Rock-2 share a structural similarity with myotonic dystrophy kinase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Rock-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 755-840 mapping within an internal region of Rock-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 755-840 mapping within an internal region of Rock-1 of human origin |
| Entrez Gene | 6093 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules.
Application
Western blot analysis of Rock-1 expression in Caki-1 (A), KNRK (B) and c4 (C) whole cell lysates and mouse liver tissue extract (D).
Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.
Application
Western blot analysis of Rock-1 expression in Hep G2 (A) and NIH/3T3 (B) whole cell lysates.| Application Notes | recommended for detection of Rock-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
