Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes and bile duct cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of MLK3 expression in non-transfected 293T: (A), mouse MLK3 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of MLK3 expression in non-transfected: (A) and human MLK3 transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing membrane staining.Application
Western blot analysis of MLK3 expression in Hep G2 (A) and Jurkat (B) whole cell lysates.| Product Name | MLK3 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of MLK3 of human origin |
| Modification Notes | As a result of the binding of growth factors to their membrane receptors, cytoplasmic proteins containing Src homology 2 (SH2) domains associate with specific phosphotyrosine residues within the activated receptors and function as signaling intermediates. The action of such SH2 domain proteins frequently involves the activation of a second group of signaling intermediates characterized by SH3 domains. These latter proteins function through binding proline-rich sequences in target proteins. A novel human non-receptor protein kinase, designated either MLK3 or PTK1, is 847 amino acids in length and contains an SH3 domain in the absence of an SH2 domain. In addition, MLK3 is characterized by a leucine zipper basic region (a motif commonly associated with transcription factors) and has a long carboxy-terminal tail which exhibits proline-rich motifs similar to known SH3 binding sites. MLK3 is expressed widely and is related to the previously described MLK1 and MLK2 kinases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MLK3 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of MLK3 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of MLK3 of human origin |
| Entrez Gene | 4296 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes and bile duct cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of MLK3 expression in non-transfected 293T: (A), mouse MLK3 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of MLK3 expression in non-transfected: (A) and human MLK3 transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing membrane staining.
Application
Western blot analysis of MLK3 expression in Hep G2 (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of MLK3 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
