Application
Antibodies tested include ERα (MC-20): , ERα (H-184): , CBP (A-22): , CBP (C-1): , p/CIP (F2): , p/CIP (M-397): , RIP140 (H-300): and HDAC3 (N-19): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Perissi et al., Cell 2004, 116: 511-526.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.Application
Western blot analysis of human recombinant ERα.| Product Name | ERα Antibody (MC-20) TRITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of ERα of mouse origin |
| Modification Notes | Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERå and ER∫, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus , though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERå and ER∫ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERå and ER∫ may be regulated by distinct mechanisms even though they share many functional characteristics. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of estrogen receptor α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of ER&alpha of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of ERα of mouse origin |
| Entrez Gene | 2099 |
|---|
Application
Antibodies tested include ERα (MC-20): , ERα (H-184): , CBP (A-22): , CBP (C-1): , p/CIP (F2): , p/CIP (M-397): , RIP140 (H-300): and HDAC3 (N-19): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Perissi et al., Cell 2004, 116: 511-526.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.
Application
Western blot analysis of human recombinant ERα.| Application Notes | recommended for detection of estrogen receptor α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
