Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cellsApplication
Antibodies tested include p-NFATc3 (Ser 169): (A,B) and NFATc3 (M-75): (C,D).Application
Antibodies tested include p-NFATc3 (H-2): (A,B) and NFATc3 (M-75): (C,D).Application
Antibodies tested include p-NFATc3 (Ser 344): (A,B,C) and NFATc3 (M-75): (D,E,F).Application
Antibodies tested include p-NFATc3 (A-1): (A,B,C) and NFATc3 (M-75): (D,E,F).Application
Antibodies tested include p-NFATc3 (Ser 265): (A,B) and NFATc3 (M-75): (C,D).Application
Western blot analysis of NFATc3 isoform expression in Ramos whole cell lysate.| Product Name | NFATc3 Antibody (M-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 321-395 mapping within an internal region of NFATc3 of mouse origin |
| Modification Notes | Members of the NFAT (nuclear factor of activated T cells) family of transcription factors are related to NFkB/Rel proteins and form cooperative complexes with the AP-1 proteins, Fos and Jun, on DNA to regulate cytokine expression in T cells. NFAT proteins are widely expressed and alternatively modified to generate splice variants, and they are localized to both the cytosol (NFATc) and to the nucleus (NFATn). NFAT1, NFAT2 and NFAT4 are predominantly expressed in immune cells, and NFAT2 and NFAT3 are expressed at high levels in cardiac tissues. In addition to activating cytokine gene transcription, NFAT2 is also implicated in cardiac valve development and NFAT3 is involved in cardiac hypertrophy. NFAT5 is detected in both immune and nonimmune cells and, like other NFAT proteins, it contains a highly conserved Rel-like binding domain that mediates NFAT proteins associating with specific consensus sequences on DNA. NFAT proteins are activated by increases in intracellular calcium, which leads to the calmodulin-dependent phosphatase, calcineurin, dephosphorylating NFAT proteins. This activating event induces a conformational change in the protein structure that exposes the nuclear localization signal and facilitates the translocation of NFAT proteins from the cytosol into the nucleus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of NFATc3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 321-395 mapping within an internal region of NFATc3 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 321-395 mapping within an internal region of NFATc3 of mouse origin |
| Entrez Gene | 4775 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells
Application
Antibodies tested include p-NFATc3 (Ser 169): (A,B) and NFATc3 (M-75): (C,D).
Application
Antibodies tested include p-NFATc3 (H-2): (A,B) and NFATc3 (M-75): (C,D).
Application
Antibodies tested include p-NFATc3 (Ser 344): (A,B,C) and NFATc3 (M-75): (D,E,F).
Application
Antibodies tested include p-NFATc3 (A-1): (A,B,C) and NFATc3 (M-75): (D,E,F).
Application
Antibodies tested include p-NFATc3 (Ser 265): (A,B) and NFATc3 (M-75): (C,D).
Application
Western blot analysis of NFATc3 isoform expression in Ramos whole cell lysate.| Application Notes | recommended for detection of NFATc3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
