Application
Western blot analysis of XBP-1 expression in THP-1 nuclear extract.Application
Western blot analysis of XBP-1 expression in non-transfected: (A) and human XBP-1 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of XBP-1 expression in BJAB (A) and Ramos (B) nuclear extracts.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.| Product Name | XBP-1 Antibody (M-186) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 76–263 of XBP-1 (X-box binding protein-1) of mouse origin |
| Modification Notes | The X-box binding protein-1 (XBP-1 or hXBP-1), also designated tax-responsive element-binding protein 5 (TREB5) in mouse and human, or hepatocarcinogenesis-related transcription factor (HTF) in rat, belongs to the basic region/leucine zipper (bZIP) family of transcription factors. XBP-1 was first characterized as a protein that binds to the HLA-DRå promoter in B cells. XBP-1 recognizes the cAMP responsive element (CRE) in enhancers of human T cell leukemia virus and major histocompatibility complex class II genes and activates transcription of these genes. It is expressed at high levels in developing bone and its levels are modulated during osteoblast development, suggesting a role in regulation of expression of osteoblast-specific genes. In addition to binding to CRE sequences, XBP-1 has been shown to bind to TPA response elements (TREs). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of XBP-1U and XBP-1S isoforms of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 76–263 of XBP-1 (X-box binding protein-1) of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 76–263 of XBP-1 (X-box binding protein-1) of mouse origin |
| Entrez Gene | 7494 |
|---|
Application
Western blot analysis of XBP-1 expression in THP-1 nuclear extract.
Application
Western blot analysis of XBP-1 expression in non-transfected: (A) and human XBP-1 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of XBP-1 expression in BJAB (A) and Ramos (B) nuclear extracts.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.| Application Notes | recommended for detection of XBP-1U and XBP-1S isoforms of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
