Application
SREBP-1 (C-20): Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of SREBP-1 expression in NIH/3T3 (A), HeLa (B) and A549 (C) nuclear extracts.Application
Western blot analysis of SREBP-1 expression in non-transfected: (A) and human SREBP-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SREBP-1 expression in NIH/3T3 nuclear extract (A) and KNRK (B), LNCaP (C) and Hep G2 (D) whole cell lysates.| Product Name | SREBP-1 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of SREBP-1 of human origin |
| Modification Notes | The low density lipoprotein (LDL) receptor mediates the endocytic uptake of cholesterol-carrying lipoproteins, thereby controlling cholesterol levels in cells and plasma. Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5' flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. A set of SRE-binding proteins (SREBPs) have been identified, including two basic helix-loop-helix leuicine zipper (bHLH-Zip) transcription factors, designated SREBP-1 and SREBP-2. SREBP-1 (also designated ADD1, for adipocyte determination and differentiation factor) is synthesized as a precursor that is attached to the nuclear envelope and endoplasmic reticulum. In sterol-depleted cells, the membrane-bound precursor is cleaved to generate a soluble NH2-terminal fragment that translocates to the nucleus to activate transcription. Sterols inhibit the cleavage of SREBP-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SREBP-1 p125 and p68 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of SREBP-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of SREBP-1 of human origin |
| Entrez Gene | 6720 |
|---|
Application
SREBP-1 (C-20): Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of SREBP-1 expression in NIH/3T3 (A), HeLa (B) and A549 (C) nuclear extracts.
Application
Western blot analysis of SREBP-1 expression in non-transfected: (A) and human SREBP-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SREBP-1 expression in NIH/3T3 nuclear extract (A) and KNRK (B), LNCaP (C) and Hep G2 (D) whole cell lysates.| Application Notes | recommended for detection of SREBP-1 p125 and p68 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
