Application
Western blot analysis of Ets-1 expression in non-transfected: (A) and mouse Ets-1 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed KNRK cells showing nuclear localization.Application
Western blot analysis of Ets-1 expression in non-transfected control (A) and Ets-1 siRNA transfected (B) Jurkat cells. Blot probed with Ets-1 (C-20): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of Ets-1 expression in KNRK nuclear extract.| Product Name | Ets-1 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Ets-1 of human origin |
| Modification Notes | Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA-binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5’-GGAA-3’ element. Evidence indicates that the DNA binding activity by Ets-1 is regulated at the level of phosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Ets-1 p54 of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Ets-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Ets-1 of human origin |
| Entrez Gene | 2113 |
|---|
Application
Western blot analysis of Ets-1 expression in non-transfected: (A) and mouse Ets-1 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing nuclear localization.
Application
Western blot analysis of Ets-1 expression in non-transfected control (A) and Ets-1 siRNA transfected (B) Jurkat cells. Blot probed with Ets-1 (C-20): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of Ets-1 expression in KNRK nuclear extract.| Application Notes | recommended for detection of Ets-1 p54 of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
