Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of Stat3 expression in non-transfected: (A) and human Stat3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Stat3 expression in K-562 (A), 3611-RF (B), NIH/3T3 (C) and KNRK (D) whole cell lysates.| Product Name | Stat3 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Stat3 of mouse origin |
| Modification Notes | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3∫ appears to be activated by both while Stat3å is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Stat3 p92 of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Stat3 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Stat3 of mouse origin |
| Entrez Gene | 6774 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of Stat3 expression in non-transfected: (A) and human Stat3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Stat3 expression in K-562 (A), 3611-RF (B), NIH/3T3 (C) and KNRK (D) whole cell lysates.| Application Notes | recommended for detection of Stat3 p92 of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
