Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with HEB (A-20), followed by PerCP-conjugated mouse anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with HEB (A-20), followed by FITC-conjugated mouse anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with HEB (A-20), followed by PerCP-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with HEB (A-20), followed by FITC-conjugated bovine anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of HEB expression in 293T (A) and SW480 (B) whole cell lysates.Application
Western blot analysis of HEB expression in non-transfected 293T: (A), human HEB transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with HEB (A-20), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.Application
Western blot analysis of HEB expression in Jurkat nuclear extract.| Product Name | HEB Antibody (A-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the C-terminus of HEB of human origin |
| Modification Notes | Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. Both homo- and hetero-oligomers of these proteins are able to distinguish very closely related E box proteins and are believed to play important roles in lineage specific gene expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISAGSChIP |
| recommended for detection of HEB of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the C-terminus of HEB of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the C-terminus of HEB of human origin |
| Entrez Gene | 6938 |
|---|
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with HEB (A-20), followed by PerCP-conjugated mouse anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with HEB (A-20), followed by FITC-conjugated mouse anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with HEB (A-20), followed by PerCP-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with HEB (A-20), followed by FITC-conjugated bovine anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of HEB expression in 293T (A) and SW480 (B) whole cell lysates.
Application
Western blot analysis of HEB expression in non-transfected 293T: (A), human HEB transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with HEB (A-20), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.
Application
Western blot analysis of HEB expression in Jurkat nuclear extract.| Application Notes | recommended for detection of HEB of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
