Application
Western blot analysis of Cdc27 expression in non-transfected: (A) and mouse Cdc27 transfected: (B) 293T whole cell lysates.Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Cdc27 (H-300), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.Application
Western blot analysis of Cdc27 expression in Jurkat (A), phorbol-treated Jurkat (B) and K-562 (C) nuclear extracts.| Product Name | Cdc27 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Cdc27 of human origin |
| Modification Notes | Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of Cdc27 of mouse and human origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Cdc27 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Cdc27 of human origin |
| Entrez Gene | 996 |
|---|
Application
Western blot analysis of Cdc27 expression in non-transfected: (A) and mouse Cdc27 transfected: (B) 293T whole cell lysates.
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Cdc27 (H-300), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
Application
Western blot analysis of Cdc27 expression in Jurkat (A), phorbol-treated Jurkat (B) and K-562 (C) nuclear extracts.| Application Notes | recommended for detection of Cdc27 of mouse and human origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
