Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Western blot analysis of c-Fos expression in non-transfected 293T: (A), mouse c-Fos transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of c-Fos expression in untreated (A) and phorbol ester-treated (B) nuclear cell extracts.Application
Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of c-Fos expression in non-transfected control (A) and c-Fos siRNA transfected (B) A-431 cells. Blot probed with c-Fos (4): . Actin (I-19): used as specificity and loading control.Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and EGF transfected (solid orange histogram) A-431 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .Application
Nuclear immunofluorescence staining of methanol-fixed, phorbol ester-induced HeLa cells.| Product Name | c-Fos Antibody (4) FITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of c-Fos of human origin |
| Modification Notes | The c-Fos oncogene was initially detected in two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. The cellular homolog, c-Fos, encodes a nuclear phospho-protein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers which bind to specific DNA response elements, c-Fos proteins are only active as heterodimers with members of the Jun gene family. Functional homologs of c-Fos include the Fra-1, Fra-2 and Fos B genes. In addition, selected ATF/CREB family members can form leucine zipper dimers with Fos and Jun. Different dimers exhibit differential specificity and affinity for AP-1 and CRE sites. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISAGSChIP |
| recommended for detection of c-Fos of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine and feline: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of c-Fos of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of c-Fos of human origin |
| Entrez Gene | 2353 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Western blot analysis of c-Fos expression in non-transfected 293T: (A), mouse c-Fos transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of c-Fos expression in untreated (A) and phorbol ester-treated (B) nuclear cell extracts.
Application
Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of c-Fos expression in non-transfected control (A) and c-Fos siRNA transfected (B) A-431 cells. Blot probed with c-Fos (4): . Actin (I-19): used as specificity and loading control.
Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and EGF transfected (solid orange histogram) A-431 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .
Application
Nuclear immunofluorescence staining of methanol-fixed, phorbol ester-induced HeLa cells.| Application Notes | recommended for detection of c-Fos of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine and feline: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
