Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.Application
Western blot analysis of CENP-E expression in untreated (A) and calyculin A-treated (B) A-431 whole cell lysates.| Product Name | CENP-E Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 2364-2663 mapping at the C-terminus of CENP-E of human origin |
| Modification Notes | A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. Centromere Protein E, CENP-E, is a kinetochore motor protein that specifies kinetochore binding in mitosis. Both CENP-E and CENP-F are expressed during mitosis where they mediate the G(2) to M phase checkpoint. CENP-E is also expressed in high levels during meiosis I and meiosis II where it localizes to the fibrous corona and outer plate of kinetochores on the meiotic chromosomes. CENP-E colocalizes with hBUBR1, a BUB-related kinase until mid-anaphase. After the first polar body emission, CENP-E localizes to the spindle-midzone, separating from hBUBR1 after mid-anaphase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CENP-E of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2364-2663 mapping at the C-terminus of CENP-E of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2364-2663 mapping at the C-terminus of CENP-E of human origin |
| Entrez Gene | 1062 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.
Application
Western blot analysis of CENP-E expression in untreated (A) and calyculin A-treated (B) A-431 whole cell lysates.| Application Notes | recommended for detection of CENP-E of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
