Application
Western blot analysis of Ral GDS expression in MIA PaCa-2 whole cell lysate.Application
Western blot analysis of Ral GDS expression in non-transfected 293T: (A), mouse Ral GDS transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | Ral GDS Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 42-110 mapping near the N-terminus of Ral GDS of human origin |
| Modification Notes | Ral A and Ral B constitute a distinct subfamily of Ras-related GTPases (i.e., GDP/GTP binding proteins) (1-3). Ral proteins are activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein (4,5). Unlike Ras proteins, Ral A and Ral B fail to induce transformed foci when activated variants are expressed in various recipient cells (1). A potential downstream target of Ral, designated Ral BP-1, has been shown to contain a Rho-GTPase-activating domain (1). This Rho-GTPase-activating domain interacts preferentially with the Rho family member Cdc42 (1). A Ras/Ral signaling pathway has been reported to mediate phospholipase D (PLD) activation by v-Src, thus indicating PLD as another downstream target of Ral A (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ral GDS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 42-110 mapping near the N-terminus of Ral GDS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 42-110 mapping near the N-terminus of Ral GDS of human origin |
| Entrez Gene | 5900 |
|---|
Application
Western blot analysis of Ral GDS expression in MIA PaCa-2 whole cell lysate.
Application
Western blot analysis of Ral GDS expression in non-transfected 293T: (A), mouse Ral GDS transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of Ral GDS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
